A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants

A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants

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A carboxylesterase (CXE) or carboxylic-ester hydrolase is an enzyme that catalyzes carboxylic ester and water into alcohol and carboxylate.In plants, CXEs have been implicated in defense, development, and secondary metabolism.We discovered a new CXE gene in Nicotiana benthamiana that is related to cactus tiki mug virus resistance.The transcriptional level of NbCXE expression was significantly increased after Tobacco mosaic virus (TMV) infection.

Transient over-expression of NbCXE inhibited TMV accumulation in N.benthamiana plants.Conversely, when the NbCXE gene 1.29x4 was silenced with a Tobacco rattle virus (TRV)-based gene silencing system, TMV RNA accumulation was increased in NbCXE-silenced plants after infection.NbCXE protein was shown to interact with TMV coat protein (CP) in vitro.

Additionally, the expressions of host defense-related genes were increased in transient NbCXE-overexpressed plants but decreased in NbCXE silenced N.benthamiana plants.In summary, our study showed that NbCXE is a novel resistance-related gene involved in host defense responses against TMV infection.